AAVS1-Bxb1-LP-v2-TC
(Plasmid
#194326)
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PurposeAAVS1 targeting construct with the Bxb1 landing pad version 2 cassette.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 194326 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBR322/pUC
- Backbone size w/o insert (bp) 5700
- Total vector size (bp) 8200
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Vector typeMammalian Expression, Cre/Lox, Synthetic Biology
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameloxP-EBFP2-attP-BleoR-lox251
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SpeciesH. sapiens (human)
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Insert Size (bp)2500
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAVS1-Bxb1-LP-v2-TC was a gift from Richard Davis (Addgene plasmid # 194326 ; http://n2t.net/addgene:194326 ; RRID:Addgene_194326) -
For your References section:
Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC lines for integrating DNA payloads. Blanch-Asensio A, van der Vaart B, Vinagre M, Groen E, Arendzen C, Freund C, Geijsen N, Mummery CL, Davis RP. Stem Cell Res. 2023 Feb;66:102991. doi: 10.1016/j.scr.2022.102991. Epub 2022 Dec 5. 10.1016/j.scr.2022.102991 PubMed 36495703
Map uploaded by the depositor.
