|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19446||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5137
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)20
MutationHIS tag inserted to create new vector
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer LNCX
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA5/FRT/TO HIS was a gift from Harm Kampinga (Addgene plasmid # 19446 ; http://n2t.net/addgene:19446 ; RRID:Addgene_19446)
For your References section:Computational analysis of the human HSPH/HSPA/DNAJ family and cloning of a human HSPH/HSPA/DNAJ expression library. Hageman J, Kampinga HH. Cell Stress Chaperones. 2008 Aug 7. ():. 10.1007/s12192-008-0060-2 PubMed 18686016