dpYPQ230-D156R-CBE1
(Plasmid
#195367)
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PurposedLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN with 3xFLAG
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 195367 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepYPQ167
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Vector typePlant Expression, CRISPR ; Gateway compatible hA3A/Y130F-XTEN plus 3xFLAG-dLbCas12a-D156R-UGI entry clone
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehA3A/Y130F-XTEN with 3xFLAG-dLbCas12a-D156R-UGI
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Tag
/ Fusion Protein
- 3xflag
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
dpYPQ230-D156R-CBE1 was a gift from Yiping Qi (Addgene plasmid # 195367 ; http://n2t.net/addgene:195367 ; RRID:Addgene_195367) -
For your References section:
CRISPR-Cas12a base editors confer efficient multiplexed genome editing in rice. Cheng Y, Zhang Y, Li G, Fang H, Sretenovic S, Fan A, Li J, Xu J, Que Q, Qi Y. Plant Commun. 2023 Apr 13:100601. doi: 10.1016/j.xplc.2023.100601. 10.1016/j.xplc.2023.100601 PubMed 37060177
