pBABE BRG1 (K->R)
(Plasmid #1960)

Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    BRG1
  • Alt name
    BAF190a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5000
  • Mutation
    K798R. Mutation in ATP binding site.
  • Entrez Gene
    SMARCA4 (a.k.a. BAF190, BAF190A, BRG1, MRD16, RTPS2, SNF2, SNF2L4, SNF2LB, SWI2, hSNF2b)
  • Tag / Fusion Protein
    • flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Vector: pBabe linearized with SalI and CIP'd. Insert: pBS-CeBRG1 (K->R) digested with SalI to liberate entire flag tagged BRG1 K->R Seq. (Kingston #981)

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE BRG1 (K->R) was a gift from Robert Kingston (Addgene plasmid # 1960)
  • For your References section:

    Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes. Sif S, Saurin AJ, Imbalzano AN, Kingston RE. Genes Dev 2001 Mar 1;15(5):603-18. 10.1101/gad.872801 PubMed 11238380