pBABE BRG1 (K->R)
- Backbone size w/o insert (bp) 5200
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)5000
MutationK798R. Mutation in ATP binding site.
Entrez GeneSMARCA4 (a.k.a. BAF190, BAF190A, BRG1, CSS4, MRD16, RTPS2, SNF2, SNF2L4, SNF2LB, SWI2, hSNF2b)
/ Fusion Protein
- flag (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
Terms and Licenses
Vector: pBabe linearized with SalI and CIP'd. Insert: pBS-CeBRG1 (K->R) digested with SalI to liberate entire flag tagged BRG1 K->R Seq. (Kingston #981)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBABE BRG1 (K->R) was a gift from Robert Kingston (Addgene plasmid # 1960)
For your References section:Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes. Sif S, Saurin AJ, Imbalzano AN, Kingston RE. Genes Dev 2001 Mar 1;15(5):603-18. 10.1101/gad.872801 PubMed 11238380
Map uploaded by the depositor.