1
Plasmid 1960: pBABE BRG1 (K->R)
  • BRG1

  • BAF190a

  • 5000

  • H. sapiens (human)

  • SMARCA4 (BAF190, BAF190A, BRG1, MRD16, RTPS2, SNF2, SNF2L4, SNF2LB, SWI2, hSNF2b)

  • flag

  • C terminal on insert

  • K798R. Mutation in ATP binding site.

  • pBABE-puro
    (Search Vector Database)

  • Mammalian Expression, Retroviral

  • 5200

  • SalI

  • No

  • SalI

  • No

  • pBABE 5' List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Puromycin

  • View sequences (3)
  • View map

  • Robert Kingston

  • UBMTA

Comments: 

Vector: pBabe linearized with SalI and CIP'd. Insert: pBS-CeBRG1 (K->R) digested with SalI to liberate entire flag tagged BRG1 K->R Seq. (Kingston #981)

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes. Sif et al (Genes Dev 2001 Mar 1;15(5):603-18. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1960" in your Materials and Methods section.

Discontinued

Addgene no longer distributes this plasmid. Contact help@addgene.org for more information