AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA
(Plasmid
#196192)
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Purposehuman synapsin promoter driven dTomato expression at human AAVS1 safe harbour locus
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196192 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneAAVS1-SA-Puro
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Vector typeZNF donor
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For this purpose, a previously generated backbone containing 2xCHS4-EF1a-tdTomato-SV40-2xCHS4 was used (Bagley et al., 2017).
Please visit https://www.biorxiv.org/content/10.1101/2022.10.07.511280v2 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA was a gift from Jürgen Knoblich (Addgene plasmid # 196192 ; http://n2t.net/addgene:196192 ; RRID:Addgene_196192) -
For your References section:
Integrated transcriptome and proteome analysis reveals posttranscriptional regulation of ribosomal genes in human brain organoids. Sidhaye J, Trepte P, Sepke N, Novatchkova M, Schutzbier M, Durnberger G, Mechtler K, Knoblich JA. Elife. 2023 Mar 29;12:e85135. doi: 10.7554/eLife.85135. 10.7554/eLife.85135 PubMed 36989136
