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pIRES-M2BIGC
(Plasmid #196565)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196565 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pIRES
  • Backbone manufacturer
    Clontech, USA
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418) ; GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GFP-alpha-CaMKII and MLS-NR2B
  • Species
    R. norvegicus (rat)
  • Mutation
    Nil
  • Entrez Gene
    Grin2b (a.k.a. GluN2B)
  • Entrez Gene
    Camk2a (a.k.a. PK2CDD, PKCCD)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES-M2BIGC was a gift from Ramakrishnapillai Omkumar (Addgene plasmid # 196565)

  • For your References section:

    A simple end-point assay for calcium channel activity. Chandrika AR, Steephan M, Raveendran Nair R, Sudarsana Devi SP, Kumar M, Paul S, Madhavan M, Omkumar RV. Cell Calcium. 2018 Sep;74:73-85. doi: 10.1016/j.ceca.2018.05.009. Epub 2018 Jun 1. 10.1016/j.ceca.2018.05.009 PubMed 29966860
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