pEGFP-C1-(I205K)alpha-CaMKII
(Plasmid
#196568)
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PurposeMammalian expression of I205K mutant of alpha-CaMKII fused to GFP
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196568 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech, USA
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418) ; GFP
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name(I205K)alpha-CaMKII
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SpeciesR. norvegicus (rat)
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MutationI205K
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Entrez GeneCamk2a (a.k.a. PK2CDD, PKCCD)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-C1-(I205K)alpha-CaMKII was a gift from Ramakrishnapillai Omkumar (Addgene plasmid # 196568) -
For your References section:
Phosphorylation status of the NR2B subunit of NMDA receptor regulates its interaction with calcium/calmodulin-dependent protein kinase II. Raveendran R, Devi Suma Priya S, Mayadevi M, Steephan M, Santhoshkumar TR, Cheriyan J, Sanalkumar R, Pradeep KK, James J, Omkumar RV. J Neurochem. 2009 Jul . 110(1):92-105. 10.1111/j.1471-4159.2009.06108.x PubMed 19453375