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Addgene

lenti-UCOE-dCas9-BFP-Zim3-KRAB
(Plasmid #196716)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 196716 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    lenti dCas9-VP64_Blast (#61425)
  • Backbone manufacturer
    Feng Zhang Lab
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers
    BFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9-TagBFP-KRAB(ZIM3)
  • Species
    S. pyogenes
  • Mutation
    D10A, N863A
  • Promoter EF1a
  • Tags / Fusion Proteins
    • Nucleoplasmin NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    contains a ubiquitous chromatin opening element and KRAB domain from ZIM3: Alerasool N, et al. An efficient KRAB domain for CRISPRi applications in human cells. Nat Methods. 2020 doi: 10.1038/s41592-020-0966-x. PMID: 33020655.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lenti-UCOE-dCas9-BFP-Zim3-KRAB was a gift from Bernhard Schmierer (Addgene plasmid # 196716 ; http://n2t.net/addgene:196716 ; RRID:Addgene_196716)