Gene/insert name: None
Fusion protein or tag: Flag
Terminal: N terminal on backbone
Fusion protein or tag: TEV
Terminal: N terminal on backbone
Fusion protein or tag: S peptide
Terminal: N terminal on backbone
Vector backbone:
pCDNA5/FRT/TOSearch Vector Database )
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size (bp):
6938
Cloning method: Gateway Cloning
Cloning site 5':
Gateway
Site destroyed during cloning:
No
Cloning site 3':
Gateway
Site destroyed during cloning:
No
5' sequencing primer:
CGA ACG CCA GCA CAT GGA CAG CG
List of Sequencing Primers
3' sequencing primer:
TGG CTG GCA ACT AGA AGG CAC AGT CGA GGC
Bacterial resistance(s)
Ampicillin and Chloramphenicol
Growth strain(s)
ccdB Survival
Growth temperature (℃):
37
Growth instructions:
ccdB cells
High or low copy:
High Copy
Selectable markers:
Hygromycin
Sequence:
View sequences (2)
Principal Investigator:
Peter Jackson
Terms and Licenses:
MTA
Addgene has sequenced a portion of this plasmid for verification.
Click here for the sequencing
result.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
19703" in your Materials and Methods section.
