Addgene - CAG-EnR Plasmid Data

Gene/insert name:
engrailed
Insert size:
953
Species:
D. melanogaster (fly)
Entrez Gene:
en (Dmel_CG9015, 153867_at, Apa, CG9015, Dmel\CG9015, EN, En, EnR, Eng, Engrailed, Engrailed/Invected, Es, V, spt)
Mutation:
Drosophila engrailed repressor domain
Vector backbone:
CAGEN
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert:
4801
Cloning site 5':
EcoRI
Site destroyed during cloning:
Yes
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
CAG-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Connie Cepko
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A core paired-type and POU homeodomain-containing transcription factor program drives retinal bipolar cell gene expression. Kim et al (J Neurosci. 2008 Jul 30. 28(31):7748-64. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19715" in your Materials and Methods section.