Addgene - pBabe puro RalA (short) wt Plasmid Data

Plasmid 19723: pBabe puro RalA (short) wt

Gene/insert name:
V-ral simian leukemia viral oncogene homolog A
Alt name:
RalA (short)
Insert size:
621
Species:
H. sapiens (human)
GenBank ID:
NM_005402
Entrez Gene:
RALA (RAL)
Mutation:
NOTE: short splice variant Long Sp. Variant: MVDYLAN Short Sp. Variant: MAAN
Vector backbone:
pBabe puro
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5169
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
Unknown
5' sequencing primer:
pBabe 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Channing Der
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Divergent roles for RalA and RalB in malignant growth of human pancreatic carcinoma cells. Lim et al (Curr Biol. 2006 Dec 19. 16(24):2385-94. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19723" in your Materials and Methods section.