pDEST-GST-FBW7 alpha
(Plasmid #197456)

• Purpose: The plasmid expresses GST-tagged FBW7 human isoform 1. Used for in vitro translation of FBW7alpha.
• Depositing Lab: Omar Khan
• Publication: Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi: 10.1038/s41467-021-22319-5.

Backbone

• Vector backbone: pDEST-565
• Vector type: Bacterial Expression ; In vitro translation

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Used mainly for in vitro translation.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: FBW7
• Alt name: hCDC4, FBW7 isoform 1
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Mutation: See depositor comments below
• GenBank ID: NM_001349798.2
• Entrez Gene: FBXW7 (a.k.a. AGO, CDC4, DEDHIL, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4)
• Promoter: T7
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7 Forward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note, plasmid contains an A871T mutation in FBW7. This mutation is outside of the WD40 propeller and is not expected to interfere with the function of the protein.