Addgene - FU-tet-o-hOct4 Plasmid Data

Plasmid 19778: FU-tet-o-hOct4

Gene/insert name:
Oct4
Insert size:
1100
Species:
H. sapiens (human)
Entrez Gene:
POU5F1 (DADB-104B20.2, OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)
Vector backbone:
FUdeltaGW
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
8400
Cloning site 5':
EcoRI
Site destroyed during cloning:
Unknown
Cloning site 3':
EcoRI
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Konrad Hochedlinger
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Comments:

From article, concerning viral infections: For a standard infection in a 35 mmdish (aprox 10^5 cells), 10 microL rtTA+5 microL factors (OCT4, SOX2, KLF4, and NANOG)+2 microL cMYC was used in an overnight infection supplemented with 6 microg/microL polybrene.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A high-efficiency system for the generation and study of human induced pluripotent stem cells. Maherali et al (Cell Stem Cell. 2008 Sep 11. 3(3):340-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19778" in your Materials and Methods section.