pTij_sg_mmu_Med1_N_terminal
(Plasmid #197870)

• Purpose: CRISPR/Cas9/sgRNA plasmid for cutting Med1 N terminal and building mEmerald/Halo-MED1 knock-in mESCs
• Depositing Lab: James Zhe Liu
• Publication: Dong et al bioRxiv 2022.10.26.513917

Backbone

• Vector backbone: pTij_sgRNA_cloning_vector
• Backbone manufacturer: self-modified from pX330-U6-Chimeric_BB-CBh-hSpCas9
• Backbone size w/o insert (bp): 9757
• Total vector size (bp): 9760
• Modifications to backbone: insert 20bp mmu Med1 sgRNA sequence
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRSP210
• Alt name: PBP; Pparbp; TRIP-2
• Species: M. musculus (mouse)
• Insert Size (bp): 20
• Entrez Gene: Med1 (a.k.a. CRSP210, DRIP205, PBP, Pparbp, TRAP220, TRIP-2, l11Jus15)
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: bbs1 (destroyed during cloning)
• 3′ cloning site: bbs1 (destroyed during cloning)
• 5′ sequencing primer: AGGGTCGGAACAGGAGAGC

Resource Information

• Supplemental Documents:
  • pTij_sg_mmu_Med1_N_terminal.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.10.26.513917v1 for bioRxiv preprint.