Addgene - pCAG-EGFP/RFP-miR-E2-4int Plasmid Data

Plasmid 19820: pCAG-EGFP/RFP-miR-E2-4int

Gene/insert name:
miRNA
Insert size:
400
Vector backbone:
pCAG-EGFP/RFP-int
(Search Vector Database)
Vector type:
Mammalian Expression, RNAi, Cre/Lox
Backbone size w/o insert (bp):
8200
Cloning site 5':
AsiSI
Site destroyed during cloning:
No
Cloning site 3':
MluI
Site destroyed during cloning:
No
5' sequencing primer:
GCTGGACATCACCTCCCACAACG List of Sequencing Primers
3' sequencing primer:
ACAGGAGGTGGGGAGCAGGAGA
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Zuoshang Xu
Terms and Licenses:
MTA
Clontech Limited Use Label License

Comments:

A fragment of ~400 bases surrounding the miRNA insertion site was amplified from the first intron of the vector pUbC-miRNA-EGFP [36] with primers 5'-GCAATGACGCGATCGCTAATGCGGGAAAGCTCTTATTCGGGT-3' (forward) and 5'-AAGGCATGACGCGTTGTTGCGGCCGCCAGAGGTCCGGCGCCTGT-3' (reverse).

miR-E2-4:
GGTACCTGCTGTTGACAGTGAGCGAGAAGTGGTGTATCTAGAGATTATAGTGAAGCCACAGATGTATAATCTCTATACACCACTTCCTGCCTACTGCCTCGGACTTCAAGGGGAATTC

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A construct with fluorescent indicators for conditional expression of miRNA. Qiu et al (BMC Biotechnol. 2008 . 8():77. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19820" in your Materials and Methods section.