pGBT9-GGA1-GAE
(Plasmid #199174)

• Purpose: Expression of GAL4 DNA-binding domain (BD)-GGA1-GAE domain fusion protein in yeast (yeast two-hybrid assays)
• Depositing Lab: Juan Bonifacino
• Publication: Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.1093/emboj/cdg015.

Backbone

• Vector backbone: pGBT9
• Backbone size w/o insert (bp): 5524
• Total vector size (bp): 5892
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GGA1-GAE domain
• Species: H. sapiens (human)
• Insert Size (bp): 378
• Entrez Gene: GGA1
• Promoter: ADH1
• Tag / Fusion Protein:
  • GAL4-DNA binding domain fragment (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: GAL4 DNA binding domain forward
• 3′ sequencing primer: GAL4 DNA binding domain reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

GAE domain: similar to the γ-adaptin ear domain
GGA1-GAE domain: residues 515-639 of human GGA1