Skip to main content
Addgene
Open Menu

BA31_pU.CAG.SaCas9-D10A.rBGpA
(Plasmid #199251)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199251 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 199251-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 199251-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pBR322
  • Backbone size w/o insert (bp) 4965
  • Total vector size (bp) 8379
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SaCas9.D10A nickase
  • Species
    Staphylococcus aureus
  • Insert Size (bp)
    3336
  • Mutation
    D10A
  • Promoter CAG promoter
  • Tags / Fusion Proteins
    • SV40 NLS (N terminal on insert)
    • Nucleoplasmin NLS (C terminal on insert)
    • 3XHA epitope (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Insert SaCas9 described in: Ran et al. Nature 2015 520:186-191.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The insert SaCas9 was modified via the introduction of the RuvC nuclease domain-disabling mutation D10A and placed in a pCAG.rBGpA expression plasmid backbone.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BA31_pU.CAG.SaCas9-D10A.rBGpA was a gift from Manuel Goncalves (Addgene plasmid # 199251 ; http://n2t.net/addgene:199251 ; RRID:Addgene_199251)

  • For your References section:

    Precise and broad scope genome editing based on high-specificity Cas9 nickases. Wang Q, Liu J, Janssen JM, Le Bouteiller M, Frock RL, Goncalves MAFV. Nucleic Acids Res. 2021 Jan 25;49(2):1173-1198. doi: 10.1093/nar/gkaa1236. 10.1093/nar/gkaa1236 PubMed 33398349
Related items:
Commonly requested with: