Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19957||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5437
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name14-3-3 Protein Beta
SpeciesH. sapiens (human)
Entrez GeneYWHAB (a.k.a. GW128, HEL-S-1, HS1, KCIP-1, YWHAA)
/ Fusion Protein
- Myc3 (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-myc3-14-3-3 Beta was a gift from Yue Xiong (Addgene plasmid # 19957 ; http://n2t.net/addgene:19957 ; RRID:Addgene_19957)
For your References section:14-3-3beta binds to and negatively regulates the tuberous sclerosis complex 2 (TSC2) tumor suppressor gene product, tuberin. Shumway SD, Li Y, Xiong Y. J Biol Chem. 2003 Jan 24. 278(4):2089-92. 10.1074/jbc.C200499200 PubMed 12468542
Map uploaded by the depositor.