pUAST ɑSynWT-EGFP
(Plasmid
#201249)
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Purposeallows the expression of the insert in Drosophila melanogaster
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 201249 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUAST
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameɑ-Synuclein
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SpeciesH. sapiens (human)
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Entrez GeneSNCA (a.k.a. NACP, PARK1, PARK4, PD1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ɑ-Synuclein fused with EGFP tag
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUAST ɑSynWT-EGFP was a gift from Pedro Domingos (Addgene plasmid # 201249 ; http://n2t.net/addgene:201249 ; RRID:Addgene_201249) -
For your References section:
alpha-Synuclein modifies mutant huntingtin aggregation and neurotoxicity in Drosophila. Pocas GM, Branco-Santos J, Herrera F, Outeiro TF, Domingos PM. Hum Mol Genet. 2015 Apr 1;24(7):1898-907. doi: 10.1093/hmg/ddu606. Epub 2014 Dec 1. 10.1093/hmg/ddu606 PubMed 25452431
