Addgene - pGL4 mIL-17 2kb RORgamma-t mut Plasmid Data

Plasmid 20127: pGL4 mIL-17 2kb RORgamma-t mut

Gene/insert name:
mIL-17
Alt name:
mIL-17 promoter
Alt name:
IL-17
Insert size:
2000
Species:
M. musculus (mouse)
GenBank ID:
AC159614
Entrez Gene:
Il17a (Ctla-8, Ctla8, IL-17, IL-17A, Il17)
Mutation:
RORgamma-t binding site in 0.9kb of 2kb promoter has been mutated
Vector backbone:
pGL4.10
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Luciferase
Backbone size w/o insert (bp):
4242
Cloning site 5':
NheI
Site destroyed during cloning:
Unknown
Cloning site 3':
BglII
Site destroyed during cloning:
Unknown
5' sequencing primer:
RVprimer3 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Warren Strober
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Interactions between the transcription factors Runx1, RORgt and Foxp3 regulate differentiation of interleukin 17-producing T cells Fuping et al (2008 Nov;9(11):1297-306. Epub 2008 Oct 12. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20127" in your Materials and Methods section.