Addgene - pPM47 (UPR-RFP CEN/ARS URA3) Plasmid Data

Plasmid 20132: pPM47 (UPR-RFP CEN/ARS URA3)

Gene/insert name:
UPR-RFP
Alt name:
4xUPRE-mCherry
Insert size:
1419
Species:
S. cerevisiae (budding yeast)
Vector backbone:
pRS316
(Search Vector Database)
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
4835
Cloning site 5':
SacII
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
URA3
Sequence: View sequences (2)
Principal Investigator:
Feroz Papa
Terms and Licenses:
MTA
Clontech Limited Use Label License

Comments:

There is a 2 base gap between depositor's sequence and Addgene sequence; per depositor this gap is in the promoter region and shouldn't significantly affect the plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Real-time redox measurements during endoplasmic reticulum stress reveal interlinked protein folding functions. Merksamer et al (Cell. 2008 Nov 28. 135(5):933-47. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20132" in your Materials and Methods section.