pCMV6-AN-DDK_WT RAD23B_delta aa 188-228 (delta UBA1 domain)
(Plasmid
#201546)
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PurposeExpresses a mutant form of human RAD23B that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 201546 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCMV6-AN-DDK
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Backbone manufacturerOrigene
- Total vector size (bp) 7033
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUV excision repair protein RAD23 homolog B
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SpeciesH. sapiens (human)
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MutationLacks residues 188-228
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Entrez GeneRAD23B (a.k.a. HHR23B, HR23B, P58)
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV6-AN-DDK_WT RAD23B_delta aa 188-228 (delta UBA1 domain) was a gift from Roger Woodgate (Addgene plasmid # 201546 ; http://n2t.net/addgene:201546 ; RRID:Addgene_201546) -
For your References section:
A novel interaction between RAD23A/B and Y-family DNA polymerases. Ashton NW, Jaiswal N, Cestari Moreno N, Semenova IV, D'Orlando DA, Teatin Latancia M, McIntyre J, Woodgate R, Bezsonova I. J Mol Biol. 2023 Nov 5:168353. doi: 10.1016/j.jmb.2023.168353. 10.1016/j.jmb.2023.168353 PubMed 37935254
