CF-Inp(D281A)
(Plasmid #20156)

• Depositing Lab: Tobias Meyer
• Publication: Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

Backbone

• Vector backbone: ECFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4733
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CFP-FKBP-Inp54p (D281A)
• Alt name: Inp54
• Species: S. cerevisiae (budding yeast)
• Mutation: Stop codon at position 331. D281A mutation.
• GenBank ID: Z74807
• Entrez Gene: INP54 (a.k.a. YOL065C)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry