pGADCg
(Plasmid #20161)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pGADT7
  • Backbone manufacturer
    Clontech
  • Backbone size (bp) 9637
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    None
  • Tags / Fusion Proteins
    • Gal4 AD (C terminal on backbone)
    • SV40 NLS (N terminal on backbone)

Cloning Information

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGADCg was a gift from Peter Uetz (Addgene plasmid # 20161)
  • For your References section:

    Improving the yeast two-hybrid system with permutated fusions proteins: the Varicella Zoster Virus interactome. T Stellberger, R Hauser, A Baiker, V R Pothineni , J Haas, P Uetz. Proteome Sci. 2010 Feb 15;8:8. 10.1186/1477-5956-8-8