- Backbone size (bp) 9637
Vector typeYeast Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Copy numberHigh Copy
/ Fusion Proteins
- Gal4 AD (C terminal on backbone)
- SV40 NLS (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGADCg was a gift from Peter Uetz (Addgene plasmid # 20161)
For your References section:Improving the yeast two-hybrid system with permutated fusions proteins: the Varicella Zoster Virus interactome. Stellberger T, Hauser R, Baiker A, Pothineni VR, Haas J, Uetz P. Proteome Sci. 2010 Feb 15;8:8. 10.1186/1477-5956-8-8
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.