pETM11-6xHis-TEV-hORF1p_K3AK4A
(Plasmid
#202585)
-
PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 202585 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepETM11
- Backbone size w/o insert (bp) 5300
- Total vector size (bp) 6369
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameORF1p
-
Alt nameORF1
-
Alt nameL1ORF1p
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1090
-
MutationChanged ORF1p Lysine 3 to Alanine, changed ORF1p Lysine 4 to Alanine
-
Tags
/ Fusion Proteins
- 6x His (N terminal on insert)
- TEV protease cleavage site (N terminal on insert)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pETM11-6xHis-TEV-hORF1p_K3AK4A was a gift from Liam Holt (Addgene plasmid # 202585 ; http://n2t.net/addgene:202585 ; RRID:Addgene_202585) -
For your References section:
Condensation of LINE-1 is critical for retrotransposition. Sil S, Keegan S, Ettefa F, Denes L, Boeke JD, Holt LJ. Elife. 2023 Apr 28;12:e82991. doi: 10.7554/eLife.82991. 10.7554/eLife.82991 PubMed 37114770
