P789_pY026_EnAsCpf1_CLYBL_T1_catRNA
(Plasmid #202760)

• Purpose: Encodes for CRISPR-Cas12a (EnAsCpf1) with gRNA for targeting the CLYBL safe harbor site
• Depositing Lab: Karl Wahlin
• Publication: Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi: 10.1038/s41536-023-00327-x.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 4963
• Total vector size (bp): 9767
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: huAsCpf1
• gRNA/shRNA sequence: ACTTCCTTACTGTTAACTTCCATA
• Species: Synthetic
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CGCAAATGGGCGGTAGGCGTG

Resource Information

• Supplemental Documents:
  • P789_pY026_EnAsCpf1_CLYBL_T1_catRNA
• A portion of this plasmid was derived from a plasmid made by: Zhang Lab (Addgene Plasmid #84741)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry