pHD22Y/PfPRMT5 deletion
(Plasmid
#203161)
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Purposefor disruption of PfPRMT5 gene
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203161 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHD22Y
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Backbone manufacturerWellems T lab
- Backbone size w/o insert (bp) 5818
- Total vector size (bp) 2151
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Modifications to backboneNo
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Vector typeMammalian Expression
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Selectable markershdhfr
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePfPRMT5 fragment
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SpeciesPlasmodium falciprum 3D7
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Insert Size (bp)1163
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GenBank IDXM_001350285.1
- Promoter No
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Tags
/ Fusion Proteins
- 3 myc tags (C terminal on backbone)
- 3' UTR of P. berghei dihydrofolate reductase/thymidylate synthase (dhfr/ts) gene (pDT)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site Not1 (not destroyed)
- 5′ sequencing primer TATCATAAAATACCACAATGTGAAG
- 3′ sequencing primer GCGGCCGCCTACCCTGAAGAAG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
We cloned the N-terminal fragment of PfPRMT5, 3 myc tags, and 3' UTR region of P. berghei dihydrofolate reductase/thymidylate synthase (dhfr/ts) gene (pDT) into pHD22Y vector. This vector is used for disruption of the PfPRMT5 gene in the malaria parasite Plasmodium falciparum by single crossover.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHD22Y/PfPRMT5 deletion was a gift from Liwang Cui (Addgene plasmid # 203161 ; http://n2t.net/addgene:203161 ; RRID:Addgene_203161) -
For your References section:
A type II protein arginine methyltransferase regulates merozoite invasion in Plasmodium falciparum. Lucky AB, Wang C, Liu M, Liang X, Min H, Fan Q, Siddiqui FA, Adapa SR, Li X, Jiang RHY, Chen X, Cui L, Miao J. Commun Biol. 2023 Jun 22;6(1):659. doi: 10.1038/s42003-023-05038-z. 10.1038/s42003-023-05038-z PubMed 37349497