pGEM4Z-T7-5'UTR-EGFP-3'UTR-A64
(Plasmid #203348)

• Purpose: Template for T7 in vitro transcription of EGFP mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail
• Depositing Labs: Christopher Grigsby, Molly Stevens
• Publication: IVT Constructs (unpublished)

Backbone

• Vector backbone: pGEM4Z
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 2820
• Total vector size (bp): 3540
• Vector type: Template for T7-mediated in vitro transcription of mRNA

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Enhanced Green Fluorescent Protein
• Alt name: EGFP
• Species: Aequorea victoria
• Insert Size (bp): 720
• Promoter: T7
• Tag / Fusion Protein:
  • Flanked by 5' and 3' UTRs from Xenopus beta globin and a 64nt polyA tail

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: PacI (not destroyed)
• 5′ sequencing primer: AATACGACTCACTATAGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Template should be linearized with SpeI for IVT to give 1038nt transcript
The construct was found to have a 74 bp polyA sequence rather than 64 bp