Addgene - pStart-K Plasmid Data

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20346: pStart-K
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Plasmid 20346: pStart-K

Gene/insert name:
pStart-K
Species:
cloning vector
GenBank ID:
EU530620
Vector backbone:
pACYC177
(Search Vector Database)
Vector type:
Mouse Targeting
Vector type:
Gateway cloning
Cloning method:
Gateway Cloning
5' sequencing primer:
TAAACTGCCAGGCATCAAACTAAGC List of Sequencing Primers
3' sequencing primer:
AGTCAGCCCCATACGATATAAGTTG
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
DB3.1
High or low copy:
Low Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Mario Capecchi
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A protocol for constructing gene targeting vectors: generating knockout mice for the cadherin family and beyond. Wu et al (Nat Protoc. 2008 . 3(6):1056-76. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20346" in your Materials and Methods section.