pAG426GAL-SLEV_NS2B-GS-NS3
(Plasmid
#203510)
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PurposeExpresses SLEV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203510 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAG426GAL-ccdB
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepoly SLEV NS2B-GS-NS3
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Alt nameSt. Louis Encephalitis Virus NS2B-GS-NS3
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Entrez GenePOLY (a.k.a. SLEV_gp1)
- Promoter GAL1
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer TCAAATGTAATAAAAGTATCAACAAAA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG426GAL-SLEV_NS2B-GS-NS3 was a gift from Alejandro Chavez (Addgene plasmid # 203510 ; http://n2t.net/addgene:203510 ; RRID:Addgene_203510)