Addgene - pCDNA3 Flag p38 beta2 Plasmid Data

Plasmid 20355: pCDNA3 Flag p38 beta2

Gene/insert name:
pDCNA3 Flag p38 beta2
Alt name:
MapK11
Insert size:
1212
Species:
H. sapiens (human)
GenBank ID:
AF031135
Entrez Gene:
MAPK11 (P38B, P38BETA2, PRKM11, SAPK2, SAPK2B, p38-2, p38Beta)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
Cloning site 5':
Hind III
Site destroyed during cloning:
No
Cloning site 3':
Xho 1
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Roger Davis
Terms and Licenses:
MTA

Comments:

There are two additional mutations in the sequence - Q22L and M198L. These mutations are correct and the sequence still functions normally.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Selective activation of p38 mitogen-activated protein (MAP) kinase isoforms by the MAP kinase kinases MKK3 and MKK6. Enslen et al (J Biol Chem. 1998 Jan 16. 273(3):1741-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20355" in your Materials and Methods section.