PJEBAN3
(Plasmid
#203682)
-
PurposeExpresses Yellow Fluorescent Protein (YFP) in Listeria monocytogenes
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 203682 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepNF8
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Erythromycin, 200 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameYellow Fluorescent Protein (YFP)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Optimized version of the Cyan- and Yellow fluorescent proteins were created as follows: The hsp60 promoter driven GFP+ [19] was amplified and cloned into the pUC18 plasmid. pUC-yfp++ was obtained by introducing the following mutations by site-directed mutagenesis (Stratagene): F46L, S65G, V68A, S72A, S175G, T203Y, whereas the pUC-cfp++, was obtained by introducing the K26R, Y66W, N146I, N164H, S175G, N212K mutations
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
PJEBAN3 was a gift from Tine Rask Licht (Addgene plasmid # 203682 ; http://n2t.net/addgene:203682 ; RRID:Addgene_203682) -
For your References section:
Construction of a multiple fluorescence labelling system for use in co-invasion studies of Listeria monocytogenes. Andersen JB, Roldgaard BB, Lindner AB, Christensen BB, Licht TR. BMC Microbiol. 2006 Oct 3;6:86. doi: 10.1186/1471-2180-6-86. 10.1186/1471-2180-6-86 PubMed 17014739
