pSB55 - pL0_CrGLK (CDS)
(Plasmid
#203903)
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PurposeMoClo compatible L0 plasmid containing the CrGLK coding sequence
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203903 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepICH41308
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Backbone manufacturerWeber et al., 2011 (DOI:10.1371/journal.pone.0016765)
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCrGLK
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SpeciesSynthetic; Catharanthus roseus var. LBE
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Insert Size (bp)1368
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MutationSilent mutation of BpiI or BsaI cut site for MoClo domestication: T>A 474 bp from start codon
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (destroyed during cloning)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSB55 - pL0_CrGLK (CDS) was a gift from Carolyn Lee-Parsons (Addgene plasmid # 203903 ; http://n2t.net/addgene:203903 ; RRID:Addgene_203903)