PEmax-mRNA
(Plasmid
#204472)
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Purposeplasmid for PEmax mRNA IVT
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 204472 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepbluescript
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Backbone manufacturerNA
- Backbone size w/o insert (bp) 3300
- Total vector size (bp) 9167
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePrime editor
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Alt namePEmax mRNA
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Specieshuman
- Promoter T7
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Tag
/ Fusion Protein
- no
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13 Rev
- 3′ sequencing primer polyA-seq (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PEmax-mRNA was a gift from Scot Wolfe (Addgene plasmid # 204472 ; http://n2t.net/addgene:204472 ; RRID:Addgene_204472) -
For your References section:
Reducing the inherent auto-inhibitory interaction within the pegRNA enhances prime editing efficiency. Ponnienselvan K, Liu P, Nyalile T, Oikemus S, Maitland SA, Lawson ND, Luban J, Wolfe SA. Nucleic Acids Res. 2023 May 29:gkad456. doi: 10.1093/nar/gkad456. 10.1093/nar/gkad456 PubMed 37246708
