pIRESpuroN3xFLAG-UFSP2 C302A
(Plasmid
#204554)
-
PurposeExpresses 3xFLAG-tagged UFSP2 C302A in mammalian cells
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 204554 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepIRESpuro3
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameUFSP2
-
SpeciesH. sapiens (human)
-
MutationC302A
-
Entrez GeneUFSP2 (a.k.a. BHD, C4orf20, SEMDDR)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pIRESpuroN3xFLAG-UFSP2 C302A was a gift from Masaaki Komatsu (Addgene plasmid # 204554 ; http://n2t.net/addgene:204554 ; RRID:Addgene_204554) -
For your References section:
The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3. Ishimura R, El-Gowily AH, Noshiro D, Komatsu-Hirota S, Ono Y, Shindo M, Hatta T, Abe M, Uemura T, Lee-Okada HC, Mohamed TM, Yokomizo T, Ueno T, Sakimura K, Natsume T, Sorimachi H, Inada T, Waguri S, Noda NN, Komatsu M. Nat Commun. 2022 Dec 21;13(1):7857. doi: 10.1038/s41467-022-35501-0. 10.1038/s41467-022-35501-0 PubMed 36543799
