pMyosin-miRFP704nano
(Plasmid
#206194)
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PurposeLC-Myosin labelled with near-infrared fluorescent protein miRFP704nano
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206194 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemiRFP704nano-LC-Myosin
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SpeciesR. norvegicus (rat), Synthetic
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Insert Size (bp)1014
- Promoter CMV
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Tag
/ Fusion Protein
- miRFP704nano (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMyosin-miRFP704nano was a gift from Vladislav Verkhusha (Addgene plasmid # 206194 ; http://n2t.net/addgene:206194 ; RRID:Addgene_206194) -
For your References section:
Development of bright red-shifted miRFP704nano using structural analysis of miRFPnano proteins. Oliinyk OS, Pletnev S, Baloban M, Verkhusha VV. Protein Sci. 2023 Jun 22:e4709. doi: 10.1002/pro.4709. 10.1002/pro.4709 PubMed 37347539