pLAMP1-miRFP704nano
(Plasmid
#206195)
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PurposeLAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent protein miRFP704nano
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206195 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepN1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLAMP1-miRFP704nano
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SpeciesR. norvegicus (rat), Synthetic
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Insert Size (bp)1716
- Promoter CMV
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Tag
/ Fusion Protein
- miRFP704nano (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLAMP1-miRFP704nano was a gift from Vladislav Verkhusha (Addgene plasmid # 206195 ; http://n2t.net/addgene:206195 ; RRID:Addgene_206195) -
For your References section:
Development of bright red-shifted miRFP704nano using structural analysis of miRFPnano proteins. Oliinyk OS, Pletnev S, Baloban M, Verkhusha VV. Protein Sci. 2023 Jun 22:e4709. doi: 10.1002/pro.4709. 10.1002/pro.4709 PubMed 37347539