Addgene - pHJ320 Plasmid Data

Plasmid 20671: pHJ320

Gene/insert name:
Moesin
Alt name:
MSN
Insert size:
1731
Species:
H. sapiens (human)
GenBank ID:
NM_002444
Entrez Gene:
MSN ()
Fusion protein or tag:
GFP
Terminal:
C terminal on backbone
Vector backbone:
pEGFP-N1
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
GTGCATAAGTCTGGCTAC List of Sequencing Primers
3' sequencing primer:
GATGAGCAGGATGAGAAT
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
DH5a
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Principal Investigator:
Stephen Shaw
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Phospholipase C-mediated hydrolysis of PIP2 releases ERM proteins from lymphocyte membrane. Hao et al (J Cell Biol. 2009 Feb 9. 184(3):451-62. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20671" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

20680: pHJ421

8946: pBS/U6 moesin siRNA

20677: pHJ322

20676: pHJ321

20681: pHJ423