pLV-REPORT (PGK) 5xGRE-NanoLuc
(Plasmid #207170)

• Purpose: GAl4 response element driven NanoLuc Lentiviral reporter
• Depositing Lab: David Bersten
• Publication: Roennfeldt et al Biomolecules. 2023 Oct 19;13(10):1545. doi: 10.3390/biom13101545.

Backbone

• Vector backbone: pLV-REPORT
• Backbone manufacturer: inhouse
• Vector type: Lentiviral, Luciferase, Synthetic Biology
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: NanoLuciferase
• Species: Synthetic
• Promoter: Gal4 response element minP
• Tag / Fusion Protein:
  • PEST (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: NA

Gene/Insert 2

• Gene/Insert name: d2eGFP
• Species: Synthetic
• Promoter: PGK
• Tag / Fusion Protein:
  • 3x SV40 NLS (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: NA

Gene/Insert 3

• Gene/Insert name: 5x GRE
• Alt name: 5x Gal RE
• Alt name: 5x gal response element
• Species: Synthetic

Cloning Information for Gene/Insert 3

• Cloning method: Gibson Cloning
• 5′ sequencing primer: NA

Resource Information

• Supplemental Documents:
  • pLV-REPORT (PGK) 5xGRE-NanoLuc [SEQUENCE CONFIRMED].ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Bersten Lab uses DH5alpha cells for transformation. Plasmid can be grown in NEB Stable but this is not required as this Lenti backbone is observed to be very stable.