pEF-IRES-puro6 gal4DBD-HIFCAD myc tag
(Plasmid
#207171)
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Purposeexpression plasmid for Gal4DBD-HIF-CAD.myc expression
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 207171 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEF-IRES-Puro
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGal4DBD-HIF1a-CAD Myc tag linker
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SpeciesH. sapiens (human), Synthetic
- Promoter EF1a
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Tag
/ Fusion Protein
- 2xMyc tag
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF-IRES-puro6 gal4DBD-HIFCAD myc tag was a gift from David Bersten (Addgene plasmid # 207171 ; http://n2t.net/addgene:207171 ; RRID:Addgene_207171) -
For your References section:
dFLASH; dual FLuorescent transcription factor Activity Sensor for Histone integrated live-cell reporting and high-content screening. Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell DL, Peet DJ, Whitelaw ML, Bersten DC. bioRxiv 2023.11.21.568191 10.1101/2023.11.21.568191
