pACYC Destination
(Plasmid #207675)

• Purpose: (Empty Backbone) Trio “destination vectors” which harbor an operon for constitutive expression of the repressors for each inducible promoter (CymR, PhlF, and VanR for cumate, DAPG and vanillate, respectively).
• Depositing Lab: Ramon Gonzalez
• Publication: Metabolic flux optimization of iterative pathways through orthogonal gene expression control: application to the β-oxidation reversal (unpublished)

Backbone

• Vector backbone: pACYC
• Modifications to backbone: pACYC backbone harbored an operon for constitutive expression of the repressors for 3 inducible promoters (CymR, PhlF, and VanR for cumate, DAPG and vanillate, respectively).
• Vector type: Bacterial Expression, Synthetic Biology
• Promoter: CymRC, PhlF, VanCC

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: suggested Chloramphenicol concentration: 34 µg/mL
• Copy number: Low Copy

Cloning Information

• Cloning method: Ligation Independent Cloning

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The Marionette Sensor Collection was a gift from Christopher Voigt (Addgene Kit #1000000137).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry