qTAG-C-Solo-mScarlet-H2BC11
(Plasmid #207756)

• Purpose: Donor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
• Depositing Lab: Laurence Pelletier
• Publication: Philip et al bioRxiv

Backbone

• Vector backbone: pUC19
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2686
• Vector type: Mammalian Expression, CRISPR ; Donor Template

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H2BC11 Homology Arms flanking a mScarlet Tag
• Alt name: H2BC11
• Species: H. sapiens (human)
• Insert Size (bp): 1863
• Entrez Gene: H2BC11 (a.k.a. H2B/r, H2BFR, H2BJ, HIST1H2BJ)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CTGCAAGGCGATTAAGTTGGGTAAC
• 3′ sequencing primer: GGCTCGTATGTTGTGTGGAATTGT

Resource Information

• Supplemental Documents:
  • qTAG-C-Solo-mScarlet-H2BC11.dna
  • 207756-H2BC11-Genomic-Design.dna
  • 207756-H2BC11-LHA.dna
  • 207756-H2BC11-RHA.dna

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 https://www.addgene.org/207755 .