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VHL-pGex2TK
(Plasmid #20790)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pGex2TK
  • Backbone manufacturer
    Pharmacia
  • Backbone size w/o insert (bp) 4959
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    - DH5alpha for propagation - BL21 or similar for expression
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    VHL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    652
  • Mutation
    VHL wt cDNA, no mutations
  • GenBank ID
    NM_000551
  • Entrez Gene
    VHL (a.k.a. HRCA1, RCA1, VHL1, pVHL)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH I (not destroyed)
  • 3′ cloning site EcoR I (not destroyed)
  • 5′ sequencing primer pGex-5'
  • 3′ sequencing primer pGex-3'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VHL-pGex2TK was a gift from William Kaelin (Addgene plasmid # 20790)
  • For your References section:

    Tumour suppression by the human von Hippel-Lindau gene product. Iliopoulos O, Kibel A, Gray S, Kaelin WG. Nat Med. 1995 Aug . 1(8):822-6. 10.1038/nm0895-822 PubMed 7585187