Lenti-EFS-NTID194-GSQ-PMLiva3MAS-P2A-BLAST
(Plasmid #208039)

• Purpose: Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; 3MAS mutant; K>R sumoylation sites, mutated SIM); selection with blasticidin
• Depositing Labs: Rosa Barrio, James Sutherland
• Publication: Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi: 10.1038/s41467-021-26807-6.

Backbone

• Vector backbone: Derived from Addgene ID# 52962 (Zhang Lab).
• Vector type: Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PML (isoform IVa; 3MAS)
• Species: H. sapiens (human)
• Mutation: 3MAS mutant: K65R, K160R and K490R; mutated SIM
• Entrez Gene: PML (a.k.a. MYL, PP8675, RNF71, TRIM19)
• Promoter: EFS
• Tag / Fusion Protein:
  • FLAG, N-terminal Split-TurboID (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• A portion of this plasmid was derived from a plasmid made by: TurboID fragments derived from Addgene ID # 107169 (Ting Lab). Lenti-EFS/P2A-blast backbone derived from Addgene ID# 52962 (Zhang Lab).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Control for PMLiva SUMO-ID experiments; 3MAS mutant lacks major SUMOylation sites and SIM domain.