pAbaMCi-BsaI_pJMP2776
(Plasmid
#208890)
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PurposeAcinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 208890 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneR6K
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Gentamicin, 100 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)BW25141
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Growth instructionspir dependent origin of replication
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namedCas9, sgRNA expression cassette
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAbaMCi-BsaI_pJMP2776 was a gift from Jason Peters (Addgene plasmid # 208890 ; http://n2t.net/addgene:208890 ; RRID:Addgene_208890) -
For your References section:
Essential gene knockdowns reveal genetic vulnerabilities and antibiotic sensitivities in Acinetobacter baumannii. Ward RD, Tran JS, Banta AB, Bacon EE, Rose WE, Peters JM. mBio. 2023 Dec 21:e0205123. doi: 10.1128/mbio.02051-23. 10.1128/mbio.02051-23 PubMed 38126769
