Addgene - pCS2-FLAG-hEcto/Tif1g-CAmut Plasmid Data

Plasmid 20903: pCS2-FLAG-hEcto/Tif1g-CAmut

Gene/insert name:
Ectodermin
Alt name:
Tif1gamma
Alt name:
Transcriptional Intermediary Factor 1 gamma
Alt name:
TRIM33
Insert size:
3700
Species:
H. sapiens (human)
Entrez Gene:
TRIM33 (ECTO, PTC7, RFG7, TF1G, TIF1G, TIF1GAMMA, TIFGAMMA)
Fusion protein or tag:
FLAG
Terminal:
N terminal on insert
Mutation:
Point mutagenesis of C125 and C128 to Alanine (RING finger mutant). Bears an additional SfiI restriction site across the mutated Cysteines. N-terminal deletion of 37 aminoacids; Ectodermin protein sequence starts with PPLTAVLE...
Vector backbone:
pCS2
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4800
Cloning site 5':
StuI
Site destroyed during cloning:
Yes
Cloning site 3':
SnaBI
Site destroyed during cloning:
Yes
5' sequencing primer:
SP6 List of Sequencing Primers
3' sequencing primer:
T3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
Subcloning of a SacI-BamHI fragment from the original pFLAG-CMV2 construct by Dr. F.J. Rauscher III
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Stefano Piccolo
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Germ-layer specification and control of cell growth by Ectodermin, a Smad4 ubiquitin ligase. Dupont et al (Cell. 2005 Apr 8. 121(1):87-99. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20903" in your Materials and Methods section.