pCAG/ATP10D-HA
(Plasmid
#209217)
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PurposeMammalian expression of ATP10D
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209217 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATP10D
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SpeciesH. sapiens (human)
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Entrez GeneATP10D (a.k.a. ATPVD)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The plasmid requires co-transfection with Addgene Plasmid 209223 to ensure the cellular localization of the encoded protein (Takatsu et al., 2011, JBC).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG/ATP10D-HA was a gift from Hye-Won Shin (Addgene plasmid # 209217 ; http://n2t.net/addgene:209217 ; RRID:Addgene_209217) -
For your References section:
ATP9B, a P4-ATPase (a putative aminophospholipid translocase), localizes to the trans-Golgi network in a CDC50 protein-independent manner. Takatsu H, Baba K, Shima T, Umino H, Kato U, Umeda M, Nakayama K, Shin HW. J Biol Chem. 2011 Nov 4;286(44):38159-38167. doi: 10.1074/jbc.M111.281006. Epub 2011 Sep 13. 10.1074/jbc.M111.281006 PubMed 21914794