Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pGL3con-miR15aBul
(Plasmid #21117)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21117 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-control
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5256
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hsa-miR-15a sensor
  • Species
    Sequences containing three hsa-miR-15a complementary sites (with mismatches)
  • Insert Size (bp)
    90

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site XbaI (destroyed during cloning)
  • 5′ sequencing primer GTACCGAAAGGTCTTACCG
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3con-miR15aBul was a gift from Joshua Mendell (Addgene plasmid # 21117 ; http://n2t.net/addgene:21117 ; RRID:Addgene_21117)
  • For your References section:

    Cell-cell contact globally activates microRNA biogenesis. Hwang HW, Wentzel EA, Mendell JT. Proc Natl Acad Sci U S A. 2009 Apr 9. ():. 10.1073/pnas.0811523106 PubMed 19359480