|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21173||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4095
Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Alt namedouble homeobox, 4
SpeciesH. sapiens (human)
Insert Size (bp)1524
MutationFirst point mutation at nucleotide 5114 (numbering according to sequence published in corresponding article): tCg to tAg. Second mutation is a single nucleotide insertion at nuc. 5479: aga to Taga.
Entrez GeneDUX4 (a.k.a. DUX4L)
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2-mkgDUX4mal*leu* was a gift from Stephen Tapscott (Addgene plasmid # 21173 ; http://n2t.net/addgene:21173 ; RRID:Addgene_21173)
For your References section:RNA Transcripts, miRNA-sized Fragments, and Proteins Produced from D4Z4 Units: New Candidates for the Pathophysiology of Facioscapulohumeral Dystrophy. Snider L, Asawachaicharn A, Tyler AE, Geng LN, Petek LM, Maves L, Miller DG, Lemmers RJ, Winokur ST, Tawil R, van der Maarel SM, Filippova GN, Tapscott SJ. Hum Mol Genet. 2009 Apr 9. ():. 10.1093/hmg/ddp180 PubMed 19359275
Map uploaded by the depositor.