Addgene - pAc5.1C-RLuc-V5His6 Plasmid Data

Plasmid 21182: pAc5.1C-RLuc-V5His6

Gene/insert name:
Renilla Luciferase
Alt name:
RLuc
Insert size:
933
Vector backbone:
pAc5.1C
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Drosophila expression
Backbone size w/o insert (bp):
5371
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
Ac5 forward primer site List of Sequencing Primers
3' sequencing primer:
BGH reverse priming site
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
Dh5 alpha or XL1-blue
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Elisa Izaurralde
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A crucial role for GW182 and the DCP1:DCP2 decapping complex in miRNA-mediated gene silencing. Rehwinkel et al (RNA. 2005 Nov . 11(11):1640-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21182" in your Materials and Methods section.